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Effects of CHL on serum biomarkers associated with cartilage degradation and ECM metabolism. SD rats were randomly divided into six groups ( n = 8/group): NC, MIA, MIA + celecoxib (PC, 10 mg/kg/day), and CHL-treated LOW (10 mg/kg/day), MID (30 mg/kg/day), and HIGH (300 mg/kg/day) groups. CHL and celecoxib were administered orally for 6 weeks, with OA induced after 2 weeks of pre-administration by intra-articular injection of MIA. Serum levels of ( A ) COMP, ( B ) CTX-II, ( C ) GAG and ( D ) <t>aggrecan</t> were measured <t>using</t> <t>ELISA</t> kits. Values are presented as the mean ± SD ( n = 8). Statistical significance is indicated by distinct letters, with different letters indicating statistically significant differences between groups. COMP, cartilage oligomeric matrix protein; CTX-II, C-terminal crosslinked telopeptide of type II collagen; and GAG, glycosaminoglycans.
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Effects of CHL on serum biomarkers associated with cartilage degradation and ECM metabolism. SD rats were randomly divided into six groups ( n = 8/group): NC, MIA, MIA + celecoxib (PC, 10 mg/kg/day), and CHL-treated LOW (10 mg/kg/day), MID (30 mg/kg/day), and HIGH (300 mg/kg/day) groups. CHL and celecoxib were administered orally for 6 weeks, with OA induced after 2 weeks of pre-administration by intra-articular injection of MIA. Serum levels of ( A ) COMP, ( B ) CTX-II, ( C ) GAG and ( D ) <t>aggrecan</t> were measured <t>using</t> <t>ELISA</t> kits. Values are presented as the mean ± SD ( n = 8). Statistical significance is indicated by distinct letters, with different letters indicating statistically significant differences between groups. COMP, cartilage oligomeric matrix protein; CTX-II, C-terminal crosslinked telopeptide of type II collagen; and GAG, glycosaminoglycans.
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Effects of CHL on serum biomarkers associated with cartilage degradation and ECM metabolism. SD rats were randomly divided into six groups ( n = 8/group): NC, MIA, MIA + celecoxib (PC, 10 mg/kg/day), and CHL-treated LOW (10 mg/kg/day), MID (30 mg/kg/day), and HIGH (300 mg/kg/day) groups. CHL and celecoxib were administered orally for 6 weeks, with OA induced after 2 weeks of pre-administration by intra-articular injection of MIA. Serum levels of ( A ) COMP, ( B ) CTX-II, ( C ) GAG and ( D ) <t>aggrecan</t> were measured <t>using</t> <t>ELISA</t> kits. Values are presented as the mean ± SD ( n = 8). Statistical significance is indicated by distinct letters, with different letters indicating statistically significant differences between groups. COMP, cartilage oligomeric matrix protein; CTX-II, C-terminal crosslinked telopeptide of type II collagen; and GAG, glycosaminoglycans.
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TE-8214 binds to HSA and SSTR2 and retains octreotide-like potency. SPR sensorgrams (response vs. time) showing concentration-dependent association/dissociation phases of TE-8214 with HSA (A) or SSTR2 (B) ; fits to a 1:1 Langmuir model yielded dissociation constants (KD) of 1.86 mM forHSA-TE-8214 and 0.80 mM for SSTR2-TE-8214. Dose-dependent reduction of intracellular cAMP levels in QGP-1 (C) and <t>AR42J</t> (D) cells after a 20-minute incubation with TE-8214 or octreotide. Dose-dependent cell viability reductions in QGP-1 (E) and AR42J (F) cells after a 3-day incubation with TE-8214 or octreotide. Data are mean ± SD; n = 3.
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Effects of CHL on serum biomarkers associated with cartilage degradation and ECM metabolism. SD rats were randomly divided into six groups ( n = 8/group): NC, MIA, MIA + celecoxib (PC, 10 mg/kg/day), and CHL-treated LOW (10 mg/kg/day), MID (30 mg/kg/day), and HIGH (300 mg/kg/day) groups. CHL and celecoxib were administered orally for 6 weeks, with OA induced after 2 weeks of pre-administration by intra-articular injection of MIA. Serum levels of ( A ) COMP, ( B ) CTX-II, ( C ) GAG and ( D ) aggrecan were measured using ELISA kits. Values are presented as the mean ± SD ( n = 8). Statistical significance is indicated by distinct letters, with different letters indicating statistically significant differences between groups. COMP, cartilage oligomeric matrix protein; CTX-II, C-terminal crosslinked telopeptide of type II collagen; and GAG, glycosaminoglycans.

Journal: Nutrients

Article Title: CHL Attenuates MIA-Induced Osteoarthritis by Regulating Inflammatory, Catabolic, and Anabolic Pathways Associated with Cartilage and Subchondral Bone Degeneration

doi: 10.3390/nu18183031

Figure Lengend Snippet: Effects of CHL on serum biomarkers associated with cartilage degradation and ECM metabolism. SD rats were randomly divided into six groups ( n = 8/group): NC, MIA, MIA + celecoxib (PC, 10 mg/kg/day), and CHL-treated LOW (10 mg/kg/day), MID (30 mg/kg/day), and HIGH (300 mg/kg/day) groups. CHL and celecoxib were administered orally for 6 weeks, with OA induced after 2 weeks of pre-administration by intra-articular injection of MIA. Serum levels of ( A ) COMP, ( B ) CTX-II, ( C ) GAG and ( D ) aggrecan were measured using ELISA kits. Values are presented as the mean ± SD ( n = 8). Statistical significance is indicated by distinct letters, with different letters indicating statistically significant differences between groups. COMP, cartilage oligomeric matrix protein; CTX-II, C-terminal crosslinked telopeptide of type II collagen; and GAG, glycosaminoglycans.

Article Snippet: ELISA kits for GAGs and aggrecan were obtained from Biomatik (Kitchener, ON, Canada).

Techniques: Injection, Enzyme-linked Immunosorbent Assay

TE-8214 binds to HSA and SSTR2 and retains octreotide-like potency. SPR sensorgrams (response vs. time) showing concentration-dependent association/dissociation phases of TE-8214 with HSA (A) or SSTR2 (B) ; fits to a 1:1 Langmuir model yielded dissociation constants (KD) of 1.86 mM forHSA-TE-8214 and 0.80 mM for SSTR2-TE-8214. Dose-dependent reduction of intracellular cAMP levels in QGP-1 (C) and AR42J (D) cells after a 20-minute incubation with TE-8214 or octreotide. Dose-dependent cell viability reductions in QGP-1 (E) and AR42J (F) cells after a 3-day incubation with TE-8214 or octreotide. Data are mean ± SD; n = 3.

Journal: Frontiers in Endocrinology

Article Title: A long-acting octreotide analog formed by conjugation with a peptide linker modified with two fatty acids

doi: 10.3389/fendo.2026.1871977

Figure Lengend Snippet: TE-8214 binds to HSA and SSTR2 and retains octreotide-like potency. SPR sensorgrams (response vs. time) showing concentration-dependent association/dissociation phases of TE-8214 with HSA (A) or SSTR2 (B) ; fits to a 1:1 Langmuir model yielded dissociation constants (KD) of 1.86 mM forHSA-TE-8214 and 0.80 mM for SSTR2-TE-8214. Dose-dependent reduction of intracellular cAMP levels in QGP-1 (C) and AR42J (D) cells after a 20-minute incubation with TE-8214 or octreotide. Dose-dependent cell viability reductions in QGP-1 (E) and AR42J (F) cells after a 3-day incubation with TE-8214 or octreotide. Data are mean ± SD; n = 3.

Article Snippet: Intracellular CgA levels in AR42J cell lysates (diluted 1:5 with Standard/Sample Diluent) was quantified by Rat CgA ELISA (RK03567, ABclonal) by measuring optical density at 450 nm.

Techniques: Concentration Assay, Incubation